Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121138_pkow ATAGTTTTATGAATATAGCTTTTATTTCAAAG(+CAGTGT)GTACCCAGCCAAAGATAATTAAAACTCTATT NGG 10 8 17 8 24 0 100 5 10
    240605121137_feue AACAAAATAGAGTTTTAATTATCTTTGGCTGG(+CAGTGT)GTACCTTTGAAATAAAAGCTATATTCATAAA NGG 10 8 17 8 24 0 100 5 10
    240605121136_mhfq AACCACAGCTCTGCTAGTCAGGACAACGTTAG(+CAGTGT)GTACCACTGACGACCCACAGGCCACTCATGC NGG 10 8 17 8 24 0 100 5 10
    240605121134_rfx6 ACTCGGCATGAGTGGCCTGTGGGTCGTCAGTG(+CAGTGT)GTACCTAACGTTGTCCTGACTAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605121132_wfht GTGATCTAAAGACACTAATGCATAGTCCTTAG(+CAGTGT)GATCCCCTAAGAAAAAGACCCCAGTTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605121130_tv5i CCTGTATTTTAACTGGGGTCTTTTTCTTAGGG(+CAGTGT)GATCCTAAGGACTATGCATTAGTGTCTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605121129_vx13 CAAAATGTTTGATAATTATTCCAGATAAAACG(+CAGTGT)GATCCTCAGAGAGCAGGTCACCACCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240605121128_yff3 TACATAGGAGTGGTGGTGACCTGCTCTCTGAG(+CAGTGT)GATCCGTTTTATCTGGAATAATTATCAAACA NGG 10 8 17 8 24 0 100 5 10
    240605121126_grgs TTGTTTGCTGTTTTCCATTTCCTGGCTCCATG(+CAGTGT)GTACCATTGTTTGCCAGGGAGCCCCACATGT NGG 10 8 17 8 24 0 100 5 10
    240605121125_4dfd GGGCCACATGTGGGGCTCCCTGGCAAACAATG(+CAGTGT)GTACCATGGAGCCAGGAAATGGAAAACAGCA NGG 10 8 17 8 24 0 100 5 10