Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121108_p6tu TTCTGTACTGCTTAAGATTCAGCAGGGCACTG(+CAGTGT)GATCCCTGGGAGCTGGAGTTAGGTGACTATG NGG 10 8 17 8 24 0 100 5 10
    240605121106_21y8 CCACTCATAGTCACCTAACTCCAGCTCCCAGG(+CAGTGT)GATCCAGTGCCCTGCTGAATCTTAAGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605121104_efsa GCATCAAGTCTACCTTTTGCATTGGTTTTATG(+CAGTGT)GATCCATGTATATAGGTAGCCATCATAAACT NGG 10 8 17 8 24 0 100 5 10
    240605121103_vuhm CCTTCAGTTTATGATGGCTACCTATATACATG(+CAGTGT)GATCCATAAAACCAATGCAAAAGGTAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605121101_2aeg CACGTGACCCTGAACAACAGTGAGCCTCTCGG(+CAGTGT)GATCCAGCCCACCATCACCTTGTTCGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605121100_r5b5 CTCATAGCTCCGAACAAGGTGATGGTGGGCTG(+CAGTGT)GATCCCGAGAGGCTCACTGTTGTTCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605121058_yl1o TGTTATTTCTTTAGCAGTGGTTAGGATGCTAG(+CAGTGT)GTACCACTGGTTTCTCTCATCAACGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605121056_penr TTTGGCTGCAGCGTTGATGAGAGAAACCAGTG(+CAGTGT)GTACCTAGCATCCTAACCACTGCTAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605121054_71pp TTAACTACATCTACAGCTATGTTTGTTTCCCG(+CAGTGT)GTACCCGGTTACAGTGAGTCTCTGCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605121052_l8s0 TTCACAGCAGGGCAGAGACTCACTGTAACCGG(+CAGTGT)GTACCGGGAAACAAACATAGCTGTAGATGTA NGG 10 8 17 8 24 0 100 5 10