Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121930_qs93 CCAAGTTCCTCAGCACTCTCTGAATGACTGTG(+CAGTGT)GATCCTTGAGGAGGAGGAGGAGCAACGCGAA NGG 10 8 17 8 24 0 100 5 10
    240605121929_ffhv CCGGGTTCGCGTTGCTCCTCCTCCTCCTCAAG(+CAGTGT)GATCCACAGTCATTCAGAGAGTGCTGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605121928_3cgd ATTCCTGAAAGAAAAGCCAAAATCACAAGCAG(+CAGTGT)GATCCTTTAAAGTCTTTGTGCTCCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240605121926_qijh GGTAAAACGCTGGGAGCACAAAGACTTTAAAG(+CAGTGT)GATCCTGCTTGTGATTTTGGCTTTTCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605121925_t7x9 TTAAGGCAAGTGTGTCATGGTGCTGAGCGTGG(+CAGTGT)GTACCAATTAGATGTTTCAGTGACACATGCT NGG 10 8 17 8 24 0 100 5 10
    240605121924_zp5s CACAAAGCATGTGTCACTGAAACATCTAATTG(+CAGTGT)GTACCCACGCTCAGCACCATGACACACTTGC NGG 10 8 17 8 24 0 100 5 10
    240605121922_b0v7 CTTGATCCTGATCCACAGTTAATATTGTTATG(+CAGTGT)GATCCTGGTCTACAAAGTGAGTTCCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240605121921_i5ho CTGGCTGTCCTGGAACTCACTTTGTAGACCAG(+CAGTGT)GATCCATAACAATATTAACTGTGGATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605121919_4n1b ATGATGTTACTGCTGCATCCAGCTTTTACATG(+CAGTGT)GATCCTAATTGAGGTCCTCATATTTGCATAG NGG 10 8 17 8 24 0 100 5 10
    240605121918_sjm8 GCCTCCTATGCAAATATGAGGACCTCAATTAG(+CAGTGT)GATCCATGTAAAAGCTGGATGCAGCAGTAAC NGG 10 8 17 8 24 0 100 5 10