Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121844_1to8 GCCAGCATGCAAGGCTCTGGGGTCAGTATCTG(+CAGTGT)GTACCAGAGGTGGGGTGGTAGGGTATAAACT NGG 10 8 17 8 24 0 100 5 10
    240605121842_b0gr CAGTTAGTTTATACCCTACCACCCCACCTCTG(+CAGTGT)GTACCAGATACTGACCCCAGAGCCTTGCATG NGG 10 8 17 8 24 0 100 5 10
    240605121841_0ppl TATTTGTAAAGACTGGGACCCTACCTGAAGGG(+CAGTGT)GATCCAACCACTCCACAGGAAGGGCTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605121839_wblu AAAATGTTTTAGCCCTTCCTGTGGAGTGGTTG(+CAGTGT)GATCCCCTTCAGGTAGGGTCCCAGTCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605121838_nuof GCAGCAGACTGGGACCCTGGGAGGGCTGGCAG(+CAGTGT)GATCCTGCTAGGGCCCTGCTTTACCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240605121837_675o CTGAAAGAGATGGTAAAGCAGGGCCCTAGCAG(+CAGTGT)GATCCTGCCAGCCCTCCCAGGGTCCCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605121835_gyil AAAAGGATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605121834_ojw9 TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240605121832_d1pn ACGAAATGGACAAATTTCTGGACAGATACCAG(+CAGTGT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240605121831_wf2s TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+CAGTGT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10