Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120902_kq2n CAACAGGAAGATCAACAGGGTCAACTAACCTG(+CAGTGT)GATCCTTGGGGCTCTCAGAGACTGAACCACC NGG 10 8 17 8 24 0 100 5 10
    240605120900_qey1 CGGTTGGTGGTTCAGTCTCTGAGAGCCCCAAG(+CAGTGT)GATCCAGGTTAGTTGACCCTGTTGATCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605120857_t60f TGGGTGGCTTACCTATAATTATAGCTCCAGGG(+CAGTGT)GATCCAATCCCCTCTTCTGGCCTCATCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605120856_pi1b TAGGTGCCTGATGAGGCCAGAAGAGGGGATTG(+CAGTGT)GATCCCCTGGAGCTATAATTATAGGTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605120854_3wia CCTAGAACACATAGGACCCGAAGATTCCTGAG(+CAGTGT)GATCCGAGAAGATTCCCGAGGACTGAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605120853_murd CGGCGTCTCCTCAGTCCTCGGGAATCTTCTCG(+CAGTGT)GATCCTCAGGAATCTTCGGGTCCTATGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605120852_z1gd GGATTGAACTCAAATTGCTAGGTCGGCAGCAG(+CAGTGT)GTACCTTTACCCACTGGACCATCTAGTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605120851_anz6 AAGGTGACAACTAGATGGTCCAGTGGGTAAAG(+CAGTGT)GTACCTGCTGCCGACCTAGCAATTTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605120849_e5fz ACAAGAAGATACCTGAGAGAAGCCATGGGGAG(+CAGTGT)GATCCAGCATTGATGGTGTAGCAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605120848_duhc GGCCTCTGGCTTCTGCTACACCATCAATGCTG(+CAGTGT)GATCCTCCCCATGGCTTCTCTCAGGTATCTT NGG 10 8 17 8 24 0 100 5 10