Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120935_47eg AGTGGCCAGAGGGAAGTGGATACGAGGGTAGG(+CAGTGT)GTACCAGGTTGGTCTGGAATTCTGTCAGATC NGG 10 8 17 8 24 0 100 5 10
    240605120933_swts ATGTTGATCTGACAGAATTCCAGACCAACCTG(+CAGTGT)GTACCCTACCCTCGTATCCACTTCCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605120932_kddz AGGATTGGAAACACACTGCCTAGACAAAAATG(+CAGTGT)GTACCCTGTGTTCTCTGCGTTCCTCACGTCT NGG 10 8 17 8 24 0 100 5 10
    240605120930_53ku TAGCCAGACGTGAGGAACGCAGAGAACACAGG(+CAGTGT)GTACCATTTTTGTCTAGGCAGTGTGTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605120928_98ci AGTACTGGCAGTAGCCAGGGAAATCCCATTTG(+CAGTGT)GATCCAAAGGCCAGCAGTTAGGAAAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605120927_6k2t TGACCTTCCTCTTTCCTAACTGCTGGCCTTTG(+CAGTGT)GATCCAAATGGGATTTCCCTGGCTACTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605120926_387k TCCAGTCAAATGTCTCATTGTTAGGTAGTGTG(+CAGTGT)GTACCCAGACCTAGAGGGTCTGTAACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240605120924_lbh5 AGTCAGGCCGGTTACAGACCCTCTAGGTCTGG(+CAGTGT)GTACCACACTACCTAACAATGAGACATTTGA NGG 10 8 17 8 24 0 100 5 10
    240605120922_5qhb GTCAAGAAAGCGTGATGGCAAAGACGCCATGG(+CAGTGT)GATCCCCTGACGTCAAGCAGACCTTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605120920_g3av ATTCTACAAACAAGGTCTGCTTGACGTCAGGG(+CAGTGT)GATCCCATGGCGTCTTTGCCATCACGCTTTC NGG 10 8 17 8 24 0 100 5 10