Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120918_0kiq GGGTTGTTAAAAATATGGAACCGAGAAAGAGG(+CAGTGT)GTACCAATTATTAGAACCAGACTCTATAAGT NGG 10 8 17 8 24 0 100 5 10
    240605120917_sn1i AAGGTACTTATAGAGTCTGGTTCTAATAATTG(+CAGTGT)GTACCCTCTTTCTCGGTTCCATATTTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605120914_bku2 AAAGTTCAGACTCCTTCCATCTCCACTCCTGG(+CAGTGT)GTACCTACTCTCTATCTTGTAGCATGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605120913_nhuf GAAAGGAACTCATGCTACAAGATAGAGAGTAG(+CAGTGT)GTACCCAGGAGTGGAGATGGAAGGAGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605120912_5u90 GAGTCAGATTAGTGGTGCTAGGAACTGAGCAG(+CAGTGT)GATCCCCAAGTTTATTTTCTAGAACCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605120910_bo0m ATTCTTTAAAGGTTCTAGAAAATAAACTTGGG(+CAGTGT)GATCCTGCTCAGTTCCTAGCACCACTAATCT NGG 10 8 17 8 24 0 100 5 10
    240605120908_a16j TATCTCATCTACTCCTGATGTGGGAATCCATG(+CAGTGT)GATCCCTGAGCTTTAATTTGTTCTTGCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605120906_e6kp TAAATCCAAGCAAGAACAAATTAAAGCTCAGG(+CAGTGT)GATCCATGGATTCCCACATCAGGAGTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605120905_mrgf CCAAAAATAAAAATAAAAAGCTAAACACTATG(+CAGTGT)GATCCTTAGCTAGAAAGCACAGAGATGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605120904_h1su TCACATCCACATCTCTGTGCTTTCTAGCTAAG(+CAGTGT)GATCCATAGTGTTTAGCTTTTTATTTTTATT NGG 10 8 17 8 24 0 100 5 10