Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121410_52gk AAGCAGACCACTTCAGGTTCTTCCAGCTTGCG(+CAGTGT)GTACCATGGCTTTGAGGGTCCTGCTGCTCGG NGG 10 8 17 8 24 0 100 5 10
    240605121409_mjog TGGGGCCGAGCAGCAGGACCCTCAAAGCCATG(+CAGTGT)GTACCGCAAGCTGGAAGAACCTGAAGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605121406_8tms ATGTCCCCAAAGCCATGGCAGGGCCTCCCTTG(+CAGTGT)GATCCTTTCATCAGGGGAAGGAGTGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605121405_a8cy CCCTTATACCTCACTCCTTCCCCTGATGAAAG(+CAGTGT)GATCCAAGGGAGGCCCTGCCATGGCTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605121403_z1go GAGGGAGTGGGATATGTTATGCGGGATCTGGG(+CAGTGT)GTACCCTGAAGACCCAGGAGTAAACCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605121401_vh6r TTATACCTCTGGTTTACTCCTGGGTCTTCAGG(+CAGTGT)GTACCCCAGATCCCGCATAACATATCCCACT NGG 10 8 17 8 24 0 100 5 10
    240605121400_k39k ATATGTAAAGGATACACTGTTTGAATGTGCTG(+CAGTGT)GTACCTGCTTTGCTTCTGGAGTTGAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240605121358_mzm2 GCACTGGTGGCTCAACTCCAGAAGCAAAGCAG(+CAGTGT)GTACCAGCACATTCAAACAGTGTATCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605121357_fgun AGTAATGGAAGCTTAGCGGCCTGGAGAAACCG(+CAGTGT)GATCCAGATATAAGCCAGCGTTCCTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605121355_dvqp CGGGTCCTGCCAGGAACGCTGGCTTATATCTG(+CAGTGT)GATCCGGTTTCTCCAGGCCGCTAAGCTTCCA NGG 10 8 17 8 24 0 100 5 10