Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121353_xr4h AGAGCAAGAGGCTGGACTTGATGGTGCCGTTG(+CAGTGT)GATCCCAGGTGCTCGCTCGCGGTTCTCATCC NGG 10 8 17 8 24 0 100 5 10
    240605121352_00h4 CAAAAGGATGAGAACCGCGAGCGAGCACCTGG(+CAGTGT)GATCCAACGGCACCATCAAGTCCAGCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605121350_bgul GGGAAGCTCATCACATTGTAAGGCTCTCACTG(+CAGTGT)GTACCACCCAGTCCTAATCAAGAATGGCATT NGG 10 8 17 8 24 0 100 5 10
    240605121348_h5lc CCAGAAATGCCATTCTTGATTAGGACTGGGTG(+CAGTGT)GTACCAGTGAGAGCCTTACAATGTGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240605121347_620l CCTCAGGCCTGATCCATCATGCCCTGGACGCG(+CAGTGT)GTACCCTAGAGCTTCTTAGCTGACTCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605121346_lywg GTGACAGCCAGAGTCAGCTAAGAAGCTCTAGG(+CAGTGT)GTACCGCGTCCAGGGCATGATGGATCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605121344_tfiw CTCAACGCAAACGAAGGGACTAGCTTATCCAG(+CAGTGT)GATCCAACGTGCGACAGGGATAGCCTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605121343_0z6v AGTGCTTTTAAGGCTATCCCTGTCGCACGTTG(+CAGTGT)GATCCTGGATAAGCTAGTCCCTTCGTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240605121341_3rt8 GAATGCAAAAATAACTGCATTTTCCTGTTCAG(+CAGTGT)GATCCAGTTGTATTTGACCATTCTAAATTAG NGG 10 8 17 8 24 0 100 5 10
    240605121340_n2h5 TTTTACTAATTTAGAATGGTCAAATACAACTG(+CAGTGT)GATCCTGAACAGGAAAATGCAGTTATTTTTG NGG 10 8 17 8 24 0 100 5 10