Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121545_u2lc CTTCCTGTACCTAAGGGCACTGGCTGGATTGG(+CAGTGT)GTACCGGGTAGCAGAAAAGAACGGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605121543_tp02 GCAGGCAGGCTCCCGTTCTTTTCTGCTACCCG(+CAGTGT)GTACCCAATCCAGCCAGTGCCCTTAGGTACA NGG 10 8 17 8 24 0 100 5 10
    240605121540_pw24 TCAAGCCTAGATTTGAGTTCTCCTTATCCCAG(+CAGTGT)GTACCTGGATTTATCCCTGCTGATTGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605121538_ujs7 AACAGACTGACAATCAGCAGGGATAAATCCAG(+CAGTGT)GTACCTGGGATAAGGAGAACTCAAATCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605121536_otgb GACCCCGTGGGTGAACACGCACCAGGGTGGGG(+CAGTGT)GTACCAGTGCTCCAGTTGGCCTACTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605121535_3o81 GAAGGGGAAGAAGTAGGCCAACTGGAGCACTG(+CAGTGT)GTACCCCCACCCTGGTGCGTGTTCACCCACG NGG 10 8 17 8 24 0 100 5 10
    240605121534_1109 TCTTGCTGAAATAAAGGTAAAGTCCACATTTG(+CAGTGT)GATCCTTTGCTCCTGCACCACTTTTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240605121533_d2mc CCAGCCAATGGAAAAGTGGTGCAGGAGCAAAG(+CAGTGT)GATCCAAATGTGGACTTTACCTTTATTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605121530_dnt6 TTTTTCTCTGAAATTCCTTGTCTCCGCCTCCG(+CAGTGT)GATCCAGGTCAGCAGCAGCCCCCAGAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605121528_2b0h CAGCCACAGCTCTGGGGGCTGCTGCTGACCTG(+CAGTGT)GATCCGGAGGCGGAGACAAGGAATTTCAGAG NGG 10 8 17 8 24 0 100 5 10