Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121648_klr5 ACTACTCTTGGCTTATATTAGACAGTAGAGTG(+CAGTGT)GTACCCCTACCCCATGGTACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605121647_tb0b GCATGTGTGTGTGTGTGTACCATGGGGTAGGG(+CAGTGT)GTACCACTCTACTGTCTAATATAAGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605121646_rx3b TACATTCAAGGACAGCCTGGGCTACAGGATAG(+CAGTGT)GATCCTATCTAAAAACAAAACAAAAATAACC NGG 10 8 17 8 24 0 100 5 10
    240605121644_au7w TTGAGGGTTATTTTTGTTTTGTTTTTAGATAG(+CAGTGT)GATCCTATCCTGTAGCCCAGGCTGTCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605121642_lki2 TGGTTTTGTGGGTGATTTTTCCATATTCCTAG(+CAGTGT)GATCCTAGTAGTTTAAGTGTGTAAATGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605121641_zk8n GAAGTCAGACATTTACACACTTAAACTACTAG(+CAGTGT)GATCCTAGGAATATGGAAAAATCACCCACAA NGG 10 8 17 8 24 0 100 5 10
    240605121640_6ach GGCTTTTCACCTGGTCTCAGCCTTGCCAGAGG(+CAGTGT)GATCCTCCAGGGTGAGAGACCTACAGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605121638_4pvw AGCTATCACACTGTAGGTCTCTCACCCTGGAG(+CAGTGT)GATCCCTCTGGCAAGGCTGAGACCAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605121637_cp6t TTTCCCAGTTTAGGAGCAGTAATGGGTGCTTG(+CAGTGT)GATCCCAGCAGAGAGCCTGTGGTGGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605121635_5c8p TACCCAAAAGCCCACCACAGGCTCTCTGCTGG(+CAGTGT)GATCCAAGCACCCATTACTGCTCCTAAACTG NGG 10 8 17 8 24 0 100 5 10