Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163947_4fv3 TCTTTCTAAAGTGACAGTGACAAACTAGGCAG(+AGGACA)GATCCACTCTGGGAGCCAAGGAGCGCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240613163944_pr03 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCGCAATACCTCTCCTGGGAATATATC NGG 10 8 17 8 24 0 100 5 10
    240613163943_901j TTCTGGATATATTCCCAGGAGAGGTATTGCGG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613163940_blod GGATGGACCATCTAGTGATTGCCATATGCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613163939_6o19 GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613163937_drfl ACAGAGTTTGAGAGCACAGAGGTGATGGGGAG(+AGGACA)GATCCAAGGTTGGAGATGATGATAGCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613163935_5t0k GCACTAGAAGGCTATCATCATCTCCAACCTTG(+AGGACA)GATCCTCCCCATCACCTCTGTGCTCTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613163934_qcfp TATAGTGCAGAATGGCTGACATGCCCATATAG(+AGGACA)GTACCAGGGGGAGGTTTTTTCACCCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240613163931_uagj TGCTTTGACAAGGGTGAAAAAACCTCCCCCTG(+AGGACA)GTACCTATATGGGCATGTCAGCCATTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613163929_o9k6 GCTGCTGCCCCAGTACAAAGCTATGGGCTTCG(+AGGACA)GTACCTCCACAGGGACCTAAGTCTAAACCCC NGG 10 8 17 8 24 0 100 5 10