Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163840_vh5o AACCATGTCAGTGGTCAATATCGAGTCAGTAG(+AGGACA)GTACCAGGCTATGTGGAAGACTGAAGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240613163839_o2zw ATCGAGTGCGCAGGTGCACCACATGCATGCAG(+AGGACA)GTACCCAGGAGGATGTTAGATTCCTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240613163837_4gua CTCCAGTTCCAAGGAATCTAACATCCTCCTGG(+AGGACA)GTACCTGCATGCATGTGGTGCACCTGCGCAC NGG 10 8 17 8 24 0 100 5 10
    240613163836_8iel TCGTCCACAGGACAGATGAGAAAATAGCTTTG(+AGGACA)GTACCTGTCCACCACCGTCCCCGCCGTCACA NGG 10 8 17 8 24 0 100 5 10
    240613163835_b7u2 CGGGGTGTGACGGCGGGGACGGTGGTGGACAG(+AGGACA)GTACCAAAGCTATTTTCTCATCTGTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613163832_efo9 AAAAATCCCCATACAAAAGTGAAGTCCCATTG(+AGGACA)GTACCCCTGCCATCATCTCCTAGTGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240613163831_ypih CACAGAATAGCCACTAGGAGATGATGGCAGGG(+AGGACA)GTACCAATGGGACTTCACTTTTGTATGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613163829_cuiu TATAATCCTGAACATGAAGTGTTTCTAAAAGG(+AGGACA)GATCCTCCTTCCCAGTAGACAGAAAAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240613163827_xfii GGCTCGACTTTTTTCTGTCTACTGGGAAGGAG(+AGGACA)GATCCCTTTTAGAAACACTTCATGTTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613163826_ezi5 TTTAGTCTTCTGCAACAACAGCCACCAAAGTG(+AGGACA)GATCCTAGGCGTTCCTGGCAGTCACCACTGG NGG 10 8 17 8 24 0 100 5 10