Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163752_iknw GGTGATACTCAGACAAAAGTGTGTTTGGTGAG(+AGGACA)GTACCCGCGTTATGTGAACATGACTTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613163750_m6be GCCATGCACAGTGGTACACATCTGCAATCCTG(+AGGACA)GTACCTGACAAGCTGAGACAGAAGGATCACA NGG 10 8 17 8 24 0 100 5 10
    240613163749_ehs5 GAACTTGTGATCCTTCTGTCTCAGCTTGTCAG(+AGGACA)GTACCAGGATTGCAGATGTGTACCACTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613163747_w0y3 GCCACCATACAGGATCACCTACAGAAAACAGG(+AGGACA)GATCCTCAAGGGAGGACAAATCCCCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240613163745_1zer ACGTTCTGTGGGGGGATTTGTCCTCCCTTGAG(+AGGACA)GATCCCTGTTTTCTGTAGGTGATCCTGTATG NGG 10 8 17 8 24 0 100 5 10
    240613163744_96cp CGGGCCCTGTCCTGCTCTCGCATTGCTTGATG(+AGGACA)GATCCTACACCAGTCTAAAAACTTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240613163743_b699 GTCTGCTTATGTAAGTTTTTAGACTGGTGTAG(+AGGACA)GATCCATCAAGCAATGCGAGAGCAGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240613163741_u8sd TAAGGCAGCAGCCCCAGGCAGCATTTAAGCGG(+AGGACA)GATCCTTAGCAAGACAGAATCCAGCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240613163740_0p81 GAAAGGGTTTTGCTGGATTCTGTCTTGCTAAG(+AGGACA)GATCCCGCTTAAATGCTGCCTGGGGCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613163739_i0z2 GATCTCGTTTACCTCCTGTTTAGTGGGCTTTG(+AGGACA)GATCCTCCCTGTTAGTTCTTCATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10