Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163810_nd69 CAGAAATCCCAACTTTCTTCACCTTGGTTACG(+AGGACA)GATCCACAGTTTGCACAGAAATAAATAATAT NGG 10 8 17 8 24 0 100 5 10
    240613163808_4beg CTTCACATAGCCACATCATAGACGTTTTTAGG(+AGGACA)GATCCCTGCTCTCCTAAATTGCTCCCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240613163806_rzzv TACTGACTCGGGGAGCAATTTAGGAGAGCAGG(+AGGACA)GATCCCTAAAAACGTCTATGATGTGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240613163804_ci9a CCTAACTCTGAGCAAGTGACTTCTGCAAAAGG(+AGGACA)GTACCCCGAAGGAAGCCATGTGGGCATCCTG NGG 10 8 17 8 24 0 100 5 10
    240613163802_ywpw CTGGACAGGATGCCCACATGGCTTCCTTCGGG(+AGGACA)GTACCCTTTTGCAGAAGTCACTTGCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613163801_tj5x TGGTCTGGACAGAGGTGTGCCCCATCCAGACG(+AGGACA)GTACCAGGATAGCAGCAGCTGCATGGCGCTA NGG 10 8 17 8 24 0 100 5 10
    240613163759_3rxd ACAGGTAGCGCCATGCAGCTGCTGCTATCCTG(+AGGACA)GTACCGTCTGGATGGGGCACACCTCTGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613163757_5e85 CTGCCCACTGGCCGTGCGCAGGGAGAACCTGG(+AGGACA)GATCCCATTCATCGGAGTGACACATCACGAT NGG 10 8 17 8 24 0 100 5 10
    240613163755_jrjl TGAGCATCGTGATGTGTCACTCCGATGAATGG(+AGGACA)GATCCCAGGTTCTCCCTGCGCACGGCCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613163753_olr4 ACAGAAGGGGAAGTCATGTTCACATAACGCGG(+AGGACA)GTACCTCACCAAACACACTTTTGTCTGAGTA NGG 10 8 17 8 24 0 100 5 10