Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164127_rqmb GACCGAGCTATGCATGGAGGAGGAGGCTGTTG(+AGGACA)GTACCATACTGAATTTCCACTGGACCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240613164125_zcpj TGACTTTAGAGGTCCAGTGGAAATTCAGTATG(+AGGACA)GTACCAACAGCCTCCTCCTCCATGCATAGCT NGG 10 8 17 8 24 0 100 5 10
    240613164124_emnx GTGGGCATTCATAGTCTAAATCCTCTAGGGAG(+AGGACA)GATCCTCTTGCCATCTGCCCTCCTCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240613164123_mqac CCTGGGCCACTGAGGAGGGCAGATGGCAAGAG(+AGGACA)GATCCTCCCTAGAGGATTTAGACTATGAATG NGG 10 8 17 8 24 0 100 5 10
    240613164121_odzw GGCAGGCAGATGAGAAACCAGTTGGACCTGTG(+AGGACA)GATCCCAGTGGTTCCCGTGAGTGGATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613164119_qc8w CCCACTAAAAATCCACTCACGGGAACCACTGG(+AGGACA)GATCCACAGGTCCAACTGGTTTCTCATCTGC NGG 10 8 17 8 24 0 100 5 10
    240613164116_901g AGCGTCCTTTAGAAAATGAAAGCATTGCCCGG(+AGGACA)GATCCGTTTTTTGAAAGAGCAGTGCAGTCGG NGG 10 8 17 8 24 0 100 5 10
    240613164115_dvia CTTAGCCGACTGCACTGCTCTTTCAAAAAACG(+AGGACA)GATCCCGGGCAATGCTTTCATTTTCTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613164113_stgk AGCACTGTAATAGTGGGTCCTAGGCAGCCACG(+AGGACA)GTACCCAGGACCACGTTCTCCAGCGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613164111_tjak GATCTGGAAAACGCTGGAGAACGTGGTCCTGG(+AGGACA)GTACCGTGGCTGCCTAGGACCCACTATTACA NGG 10 8 17 8 24 0 100 5 10