Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120043_hded CAGGGACATAGGAATATTACGGCTCTGCCAGG(+CAGTGT)GATCCCCCTCCCTGCTCTCACCCTTTCCGAT NGG 10 8 17 8 24 0 100 5 10
    240605120042_wodj TTTTAAACCATCAAGTGGTGCTGGGGTGTGAG(+CAGTGT)GATCCCTCCAACCCCCGGTGACTATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605120040_om7y GTGAGCCTATTATAGTCACCGGGGGTTGGAGG(+CAGTGT)GATCCTCACACCCCAGCACCACTTGATGGTT NGG 10 8 17 8 24 0 100 5 10
    240605120039_wswl TATGGATATTAGTCACAGCTCTCCCTGGATAG(+CAGTGT)GATCCATTGACCGTCTTGTTCTAACAACTTG NGG 10 8 17 8 24 0 100 5 10
    240605120038_cjvv ATTTCCAAGTTGTTAGAACAAGACGGTCAATG(+CAGTGT)GATCCTATCCAGGGAGAGCTGTGACTAATAT NGG 10 8 17 8 24 0 100 5 10
    240605120037_wb1h TGAATGTATGTTTTTCCTGTGTGTATCTGTGG(+CAGTGT)GTACCTCATGTGTATGCAGTACCCTTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605120035_i1a8 GCTGGTTTCCAAGGGTACTGCATACACATGAG(+CAGTGT)GTACCCACAGATACACACAGGAAAAACATAC NGG 10 8 17 8 24 0 100 5 10
    240605120033_tu56 TAGTGTTCTAAGGCAACAGCTTCTCCCCACAG(+CAGTGT)GATCCTTCTCCCTCGGCCTTGCTTGGCTACT NGG 10 8 17 8 24 0 100 5 10
    240605120032_whk8 ACACAAGTAGCCAAGCAAGGCCGAGGGAGAAG(+CAGTGT)GATCCTGTGGGGAGAAGCTGTTGCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605120031_om4r GAAGTCAGAGCCCACTTGTCTCCTGGGTACAG(+CAGTGT)GTACCTCATAATGGTCCTGAGATCCAAGGAG NGG 10 8 17 8 24 0 100 5 10