Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115958_ffvv CAAGGACCCCTGGGGATCCTGCCAAGCCTCAG(+CAGTGT)GTACCTCCTCCTTCCCTTTGGTCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605115956_zez6 GGACCTGGATGTTTCAGGCAATCTGCTGCCTG(+CAGTGT)GTACCTATTACTGTCACTATCCCCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605115955_59rl GGCCACCATGCTGGGGATAGTGACAGTAATAG(+CAGTGT)GTACCAGGCAGCAGATTGCCTGAAACATCCA NGG 10 8 17 8 24 0 100 5 10
    240605115953_8331 CATGTGTGCCTGGTGCCAGAGGTCAGAAAAGG(+CAGTGT)GTACCACCTCCCCTGGTACTGGAATTTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605115952_oj7b GAACTACCTAAATTCCAGTACCAGGGGAGGTG(+CAGTGT)GTACCCTTTTCTGACCTCTGGCACCAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605115950_tw81 TGGAAAAGCTTGGCAGGCCCCGGGAGGACTCG(+CAGTGT)GATCCATCCCTTTATCTCATCCCTACGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605115948_658q ATACTGGAGCGTAGGGATGAGATAAAGGGATG(+CAGTGT)GATCCGAGTCCTCCCGGGGCCTGCCAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605115947_g5s5 ATCTGTCCCATTTCAGAGAGGGCCCTTACTAG(+CAGTGT)GTACCTTTGGTGTGGGGGGCGCTTGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605115945_674s AGCTTGTTCCCCAAGCGCCCCCCACACCAAAG(+CAGTGT)GTACCTAGTAAGGGCCCTCTCTGAAATGGGA NGG 10 8 17 8 24 0 100 5 10
    240605115944_h8im GGATGTAAACACACTCTGTAAGCTGAAATTCG(+CAGTGT)GTACCCAAATGCATAAGTGGACTTATGACCA NGG 10 8 17 8 24 0 100 5 10