Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120029_1wj7 TGCTGCTCCTTGGATCTCAGGACCATTATGAG(+CAGTGT)GTACCTGTACCCAGGAGACAAGTGGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605120027_8ts0 CACAGTGAGACCTTGTCTCAAAAAGAGAGGGG(+CAGTGT)GTACCACTAACACCCACCTGGAGGTTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605120026_uov6 TTTACTTCAGAACCTCCAGGTGGGTGTTAGTG(+CAGTGT)GTACCCCCTCTCTTTTTGAGACAAGGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605120024_hu4l TAATCCCAGCGTTGGGGAAACAGAGCTAGAGG(+CAGTGT)GATCCCCATGAGTCCAAGGCTACCCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605120022_g4us TATATAAACCAGGGTAGCCTTGGACTCATGGG(+CAGTGT)GATCCCTCTAGCTCTGTTTCCCCAACGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605120021_88k8 GATGTCTGGAGAGCAAATCCCCAATAGGCTTG(+CAGTGT)GATCCTCAGAACCGGTCACCGACCCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605120020_ozo1 TGGCTCTTCCTGGGTCGGTGACCGGTTCTGAG(+CAGTGT)GATCCAAGCCTATTGGGGATTTGCTCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605120018_rhrl GGGTAGGAATTGAAGACATGGTCACACCTCAG(+CAGTGT)GTACCAGTTTCTGCTCTGCCAGGCGCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605120017_h931 AGTGTAGAAAGCGCCTGGCAGAGCAGAAACTG(+CAGTGT)GTACCTGAGGTGTGACCATGTCTTCAATTCC NGG 10 8 17 8 24 0 100 5 10
    240605120015_e7qu GTACTCCGGGCTGCCCCAGAAACCTGCCTTTG(+CAGTGT)GATCCATCTTGTCTGTTTAGAGAGAGCGGAG NGG 10 8 17 8 24 0 100 5 10