Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115348_8c70 ACGTACGTGGGCATTGCACCAAGCCTGCCCTG(+CAGTGT)GTACCAGATCTTTCTTTTCAAAGTCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605115346_pdyz GGTGTTGCACTCCAACACTTTTTACTATTCCG(+CAGTGT)GATCCCAGCAAAGGTACACATCAGCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605115344_dkc7 ATTGATTTCCTGCTGATGTGTACCTTTGCTGG(+CAGTGT)GATCCGGAATAGTAAAAAGTGTTGGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605115343_5x2r CCTGACTTCCAGTTAAGATGGAAGATTAGAAG(+CAGTGT)GTACCTCTCCTTGTGACCCACTTAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605115342_57gt GCAGTTTCACCTTAAGTGGGTCACAAGGAGAG(+CAGTGT)GTACCTTCTAATCTTCCATCTTAACTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605115341_1xqs AGCAAGGAAGGGCTAGCCACCATCTTGAGAAG(+CAGTGT)GTACCTGTTGTTCAAAACTAACTCACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605115339_ps1t ACCCCCCAGAGTGAGTTAGTTTTGAACAACAG(+CAGTGT)GTACCTTCTCAAGATGGTGGCTAGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605115337_inu2 TTTCCCTCAGTGATGAAGAGGCCCCACTGTGG(+CAGTGT)GTACCAACCAACCCTGATACATAAAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240605115336_7374 TTCCCTGTGACTTTATGTATCAGGGTTGGTTG(+CAGTGT)GTACCCACAGTGGGGCCTCTTCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605115335_4fz0 ACTTCACAAGAAGCTCTCTGCTCTCTGCACTG(+CAGTGT)GTACCCAACTATTCAGCTAATCTTCACTTCC NGG 10 8 17 8 24 0 100 5 10