Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114433_oemh TTCCTGACTTCCAGCCTTGCCTGACTCACCGG(+CAGTGT)GATCCTTATGTTTGGGCCTTCCCCATCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605114432_frm3 GTCCAATAGGATGGGGAAGGCCCAAACATAAG(+CAGTGT)GATCCCGGTGAGTCAGGCAAGGCTGGAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605114430_r94f CTGAGATTACAGAAGTACCCAGAAGTGTCTGG(+CAGTGT)GATCCCTGTCACATCTGTGTCATGTTGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605114429_4axr TTCAAATAACAACATGACACAGATGTGACAGG(+CAGTGT)GATCCCAGACACTTCTGGGTACTTCTGTAAT NGG 10 8 17 8 24 0 100 5 10
    240605114427_xmlj TGATAGCCAACCTTGGTTTAGGCATCTCTCAG(+CAGTGT)GTACCTACAATTTCCCAAGTTCCAATAAATT NGG 10 8 17 8 24 0 100 5 10
    240605114426_rk4p AACAGAATTTATTGGAACTTGGGAAATTGTAG(+CAGTGT)GTACCTGAGAGATGCCTAAACCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605114424_40pv GACAAAATAGCCCATGGAAGCACAGTGAAGGG(+CAGTGT)GTACCCTGACAGACAGATGAGCCACAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605114423_rl92 TTGAAGGGCCTGTGGCTCATCTGTCTGTCAGG(+CAGTGT)GTACCCCTTCACTGTGCTTCCATGGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240605114421_nysb CAGATGCCCTGGAACTGGAGTTACAGACTGGG(+CAGTGT)GATCCTCTGGAAGAGCAGCCAAGGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605114419_g5kn AGTGGTTAAGAGCCTTGGCTGCTCTTCCAGAG(+CAGTGT)GATCCCCAGTCTGTAACTCCAGTTCCAGGGC NGG 10 8 17 8 24 0 100 5 10