Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114416_56b0 TTCACACTAAGTAAGGGTTAAACCAGTCCCTG(+CAGTGT)GATCCTTAGTGAACTATAGAGGAGGGAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605114415_pn8k CTCAAGTACTCCCTCCTCTATAGTTCACTAAG(+CAGTGT)GATCCAGGGACTGGTTTAACCCTTACTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605114412_q2id TTCCTCATGGTCTCCTCCTACATCAAGCCAGG(+CAGTGT)GTACCCTCAATCCCCACTTTTGGCTCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605114411_ee84 CTGGGCAGAAGAGCCAAAAGTGGGGATTGAGG(+CAGTGT)GTACCCTGGCTTGATGTAGGAGGAGACCATG NGG 10 8 17 8 24 0 100 5 10
    240605114409_0p14 GTTGGTTTTGTTGTTTGTTTTTTCCGACGTTG(+CAGTGT)GTACCTTGGAAAGGTTGTGTATTTTAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605114407_65w3 TTAGTGACTTTAAAATACACAACCTTTCCAAG(+CAGTGT)GTACCAACGTCGGAAAAAACAAACAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605114406_hpfn GCCCTCGTCAGAACAGAGCCCCACCACTGCTG(+CAGTGT)GTACCCGCATGGATGATGTGTGTGAATTGCC NGG 10 8 17 8 24 0 100 5 10
    240605114404_kh4b TACTTGGCAATTCACACACATCATCCATGCGG(+CAGTGT)GTACCAGCAGTGGTGGGGCTCTGTTCTGACG NGG 10 8 17 8 24 0 100 5 10
    240605114402_q3jn AAAGTGTACATCAAGACCAACAACATCCTGTG(+CAGTGT)GTACCGCAGGATCCAGGAAAGCAGCCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605114400_zhd7 TGTCCACACAGGCTGCTTTCCTGGATCCTGCG(+CAGTGT)GTACCACAGGATGTTGTTGGTCTTGATGTAC NGG 10 8 17 8 24 0 100 5 10