Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114313_jmet GCCTCCGGGCTAATCCCAAATAAAACCTTTGG(+CAGTGT)GTACCAAGCAAAAGTTCTAGCTCTTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605114311_4411 GGTAGCTCACCAAGAGCTAGAACTTTTGCTTG(+CAGTGT)GTACCCAAAGGTTTTATTTGGGATTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240605114310_kfc5 GCCTCCGGGCTAATCCCAAATAAAACCTTTGG(+CAGTGT)GTACCAAGCAAAAGTTCTAGCTCTTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605114308_lz1n GGTAGCTCACCAAGAGCTAGAACTTTTGCTTG(+CAGTGT)GTACCCAAAGGTTTTATTTGGGATTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240605114307_mg20 ACCCCAGGAACTCCTAAACAGTTTAGGAGCTG(+CAGTGT)GATCCTCCCACTCTGGGATGACCCAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240605114305_5a82 TCGGGAACATTTGGGTCATCCCAGAGTGGGAG(+CAGTGT)GATCCAGCTCCTAAACTGTTTAGGAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605114303_bu4r CATCAGGACAAGATTTGTATAGGAATGCTGGG(+CAGTGT)GATCCAAACTCAAGTGTACTTATACCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605114302_t0wp GGACTTGGTTGGTATAAGTACACTTGAGTTTG(+CAGTGT)GATCCCCAGCATTCCTATACAAATCTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605114301_9gfr CAAACAGTTTAATAGCAAATAAACAAAGGAAG(+CAGTGT)GTACCACACTTGGCAGATACAAAATGACTTT NGG 10 8 17 8 24 0 100 5 10
    240605114259_vllj GACAAAAAGTCATTTTGTATCTGCCAAGTGTG(+CAGTGT)GTACCTTCCTTTGTTTATTTGCTATTAAACT NGG 10 8 17 8 24 0 100 5 10