Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114258_hgxl TGTACTCCCTGAGCAGAGCAGCCAGCCTCCTG(+CAGTGT)GATCCCCCACTGAGACCTGGAGGCCACCACA NGG 10 8 17 8 24 0 100 5 10
    240605114255_c95x CTCTTTGTGGTGGCCTCCAGGTCTCAGTGGGG(+CAGTGT)GATCCAGGAGGCTGGCTGCTCTGCTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605114253_flbq TCCCTGCATGTATGCCTGCAGGTCAGAAGAGG(+CAGTGT)GTACCAGATCCCGTTACAGATGGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605114252_wh7j TGGTGGCTCACAACCATCTGTAACGGGATCTG(+CAGTGT)GTACCCTCTTCTGACCTGCAGGCATACATGC NGG 10 8 17 8 24 0 100 5 10
    240605114250_g2os AGTAGGCTAGTCTGGCTGGCCAGCACCACAGG(+CAGTGT)GATCCTGTCTCTACCCTCCAGCATGGGCATC NGG 10 8 17 8 24 0 100 5 10
    240605114249_bx5b CTTGTGATGCCCATGCTGGAGGGTAGAGACAG(+CAGTGT)GATCCCTGTGGTGCTGGCCAGCCAGACTAGC NGG 10 8 17 8 24 0 100 5 10
    240605114247_qbed CCTGGCAGACCCTAAGGGTTTTAGGCAGCTGG(+CAGTGT)GATCCAGTGGGGAGGGCTGGCAAAGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605114246_dplf TAATTCCTTTCCTTTGCCAGCCCTCCCCACTG(+CAGTGT)GATCCCAGCTGCCTAAAACCCTTAGGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605114244_2e52 AGGACATCCACATACATAGTGCTCAATTCTTG(+CAGTGT)GTACCAGAAGCAACCTTATTCTACTTACTGA NGG 10 8 17 8 24 0 100 5 10
    240605114243_ly3f ATTTGTCAGTAAGTAGAATAAGGTTGCTTCTG(+CAGTGT)GTACCAAGAATTGAGCACTATGTATGTGGAT NGG 10 8 17 8 24 0 100 5 10