Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115135_ejd1 TCTGTTTGCACATCAAGCCAAGGAACCAATGG(+CAGTGT)GATCCTAGCTGGACTTTCCCCAGCACTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605115133_wyg1 AGTGTGTGTTCCTCCTGGAAGTAGAAAGAGAG(+CAGTGT)GATCCGGGACAAATCCAGACAAGTCCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605115131_bkwp ACATACCCCTGGACTTGTCTGGATTTGTCCCG(+CAGTGT)GATCCTCTCTTTCTACTTCCAGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605115129_1iry GCTTGACAGTGTCACCCAGTGGTGACACTAGG(+CAGTGT)GATCCTGGTACACAGGGCACACCTTTCACTG NGG 10 8 17 8 24 0 100 5 10
    240605115127_moy8 CCTCACAGTGAAAGGTGTGCCCTGTGTACCAG(+CAGTGT)GATCCCTAGTGTCACCACTGGGTGACACTGT NGG 10 8 17 8 24 0 100 5 10
    240605115126_jq0u TAGAAACATAAAACCAGATACATGCAAATGAG(+CAGTGT)GATCCTAACTATATTCTCAGCCACAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605115125_6ixr AGAAGAGCCTCTGTGGCTGAGAATATAGTTAG(+CAGTGT)GATCCTCATTTGCATGTATCTGGTTTTATGT NGG 10 8 17 8 24 0 100 5 10
    240605115123_lpvr TGGCCCAGCGTATGCACTGGCACTGGGAAGGG(+CAGTGT)GATCCAGAAGCGGTAGCTGAAGGAGAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605115121_d2fu CGGGACAGTCTCTCCTTCAGCTACCGCTTCTG(+CAGTGT)GATCCCCTTCCCAGTGCCAGTGCATACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605115120_ev4c GTGACGCTGGGTGCTGGAAGTAGCAGCTGAGG(+CAGTGT)GATCCTTCTTGGTCCTCAGGATTCTGGGAAT NGG 10 8 17 8 24 0 100 5 10