Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114142_o6pa AAACCCAAAGCATACGCAGGAGTCAGTTCCTG(+CAGTGT)GTACCGTAGAGTGAGAAAGACACCTGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605114140_6by5 CTTCCCTGCACAGGTGTCTTTCTCACTCTACG(+CAGTGT)GTACCAGGAACTGACTCCTGCGTATGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605114139_e4vt CAGGGTAGGTGTAGTTCTGAGAGTTGTGAGAG(+CAGTGT)GATCCAAAGATCACTTGGGCAGGTTTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605114138_0d0e AAATCTACTCAAACCTGCCCAAGTGATCTTTG(+CAGTGT)GATCCTCTCACAACTCTCAGAACTACACCTA NGG 10 8 17 8 24 0 100 5 10
    240605114137_qf6b TGAAGACAGCCCATTTCACAGACAATGACATG(+CAGTGT)GTACCCAGCAGCATCTCCCCTTCTGCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605114135_rbnz GTCCTTACAAGCAGAAGGGGAGATGCTGCTGG(+CAGTGT)GTACCATGTCATTGTCTGTGAAATGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605114134_3vpi AGGGATGTGTTCTATGGCCTGTGTGCTTTGTG(+CAGTGT)GTACCACCCAGCCCAGGCAGAAACTGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605114132_i7ot TAACAAAGCCCAGTTTCTGCCTGGGCTGGGTG(+CAGTGT)GTACCACAAAGCACACAGGCCATAGAACACA NGG 10 8 17 8 24 0 100 5 10
    240605114131_m9us TTTTTTTAAAAAAGAAACATGATTATGCATAG(+CAGTGT)GATCCATTTACATAATGCAAATAAGCTTTCG NGG 10 8 17 8 24 0 100 5 10
    240605114130_x6th GAGAACGAAAGCTTATTTGCATTATGTAAATG(+CAGTGT)GATCCTATGCATAATCATGTTTCTTTTTTAA NGG 10 8 17 8 24 0 100 5 10