Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114128_v7cy TTTAAGGAATATGGTTGCAGCAAAACAGAAAG(+CAGTGT)GATCCAAAAGTGGTTAGGAGCCTTGTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605114127_b76e AGTGCATTAGACAAGGCTCCTAACCACTTTTG(+CAGTGT)GATCCTTTCTGTTTTGCTGCAACCATATTCC NGG 10 8 17 8 24 0 100 5 10
    240605114126_4a2o AACAAGAAAATTTGCTTGCTAACTCTTCACTG(+CAGTGT)GTACCAGAATATTTTTAGAGCTAAAATGCCA NGG 10 8 17 8 24 0 100 5 10
    240605114125_p9wt CAGCTTGGCATTTTAGCTCTAAAAATATTCTG(+CAGTGT)GTACCAGTGAAGAGTTAGCAAGCAAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240605114124_b14b CTTTCCATCCTGAACCCATACTGCTGGATTTG(+CAGTGT)GATCCATGGACCTGAGGGCCATCAGCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605114122_0g6m CAGGAGTGGGGCTGATGGCCCTCAGGTCCATG(+CAGTGT)GATCCAAATCCAGCAGTATGGGTTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605114120_gxp3 CAATTTTAATGTTTCCTAAGCATTTTGACCAG(+CAGTGT)GTACCCAGGTTTAAGCTATGAACATTGACAG NGG 10 8 17 8 24 0 100 5 10
    240605114118_cn92 GGACACTGTCAATGTTCATAGCTTAAACCTGG(+CAGTGT)GTACCTGGTCAAAATGCTTAGGAAACATTAA NGG 10 8 17 8 24 0 100 5 10
    240605114116_vjxy CCTTGAGACCTTTATAAGCAAAAGCAAACCAG(+CAGTGT)GTACCCACCAGTTTGCTCAGACCCAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605114114_xnem ACAAAGTCTGCTGGGTCTGAGCAAACTGGTGG(+CAGTGT)GTACCTGGTTTGCTTTTGCTTATAAAGGTCT NGG 10 8 17 8 24 0 100 5 10