Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114029_tael TTGATATTTAGATTTAGCAAATAAAAATCTAG(+CAGTGT)GTACCTTGAGATAGTGAGGCATGTTGCAATC NGG 10 8 17 8 24 0 100 5 10
    240605114027_a5me GTACACTCTTCAGGACCACCTGTCTAGGGCTG(+CAGTGT)GTACCAGCCACAAGGGCTGCTTCCTCCCACA NGG 10 8 17 8 24 0 100 5 10
    240605114026_u7a3 ATTGATGTGGGAGGAAGCAGCCCTTGTGGCTG(+CAGTGT)GTACCAGCCCTAGACAGGTGGTCCTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605114024_zxvx AATCCTTTGAGTGAACCTAGTGTTTCCGGCTG(+CAGTGT)GATCCTGAGAGATACAAATGGAAAAATACTG NGG 10 8 17 8 24 0 100 5 10
    240605114023_dt4c CAGGTCAGTATTTTTCCATTTGTATCTCTCAG(+CAGTGT)GATCCAGCCGGAAACACTAGGTTCACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605114021_wr43 AGGATACTTAAGTCATCAAAGACATTGGTATG(+CAGTGT)GATCCGCTGTGGTGGTACATGCTGTAATCTC NGG 10 8 17 8 24 0 100 5 10
    240605114020_kgro GTGCTGAGATTACAGCATGTACCACCACAGCG(+CAGTGT)GATCCATACCAATGTCTTTGATGACTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605114019_fgpe AGCACTGGGCTCGCTTGGCATGTCCTGGGTTG(+CAGTGT)GATCCCAGTACTCCAACTGAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605114017_iqfe CTTTTTTTTTTTTTTTTCAGTTGGAGTACTGG(+CAGTGT)GATCCAACCCAGGACATGCCAAGCGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605114016_9nx1 GCAAGCTGCTTCCCCCGAGCCTTCAGCACAGG(+CAGTGT)GATCCTCTCAACCACAGGCAGCCGGGTTGCC NGG 10 8 17 8 24 0 100 5 10