Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113924_31ey AGTTGATTTGGTGAATGACAGTCCACTTCCTG(+CAGTGT)GATCCTCAGAGATGTTTCTTTCAGTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605113922_8p7m TAATTTTTTGTAACTTTCCACTACTCTACCTG(+CAGTGT)GTACCATATCTGTGGATTTTGGCCAATATTA NGG 10 8 17 8 24 0 100 5 10
    240605113920_5gxd AGTTTTAATATTGGCCAAAATCCACAGATATG(+CAGTGT)GTACCAGGTAGAGTAGTGGAAAGTTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605113919_twij TGTGGCAGGGTGGATAATGCGTGAGGACTTGG(+CAGTGT)GATCCTGATGGACTTTTAATTGTACCCACAA NGG 10 8 17 8 24 0 100 5 10
    240605113917_xnet TTGAGTTGTGGGTACAATTAAAAGTCCATCAG(+CAGTGT)GATCCCAAGTCCTCACGCATTATCCACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605113916_nkfh AATCCTCTCTACTTGACTCAGGGGCTCACAAG(+CAGTGT)GTACCATTATATGCAAAAGTACTGTATATTT NGG 10 8 17 8 24 0 100 5 10
    240605113915_7eey AGGAAAAATATACAGTACTTTTGCATATAATG(+CAGTGT)GTACCTTGTGAGCCCCTGAGTCAAGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605113913_jir8 CTAAATTTAAAAGTCTTAATGAATCTCCTTTG(+CAGTGT)GATCCCTGAATGGTCCAAGATCTTGGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605113911_9fq5 GCTCCAGACTCCAAGATCTTGGACCATTCAGG(+CAGTGT)GATCCAAAGGAGATTCATTAAGACTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605113909_5w00 AAAGTATGAATGTGTTCTGCACAGCAAACTGG(+CAGTGT)GATCCTTATCAGTACAACCTGAAGACTAGAG NGG 10 8 17 8 24 0 100 5 10