Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113853_awxu GAGTTTCATAAAGCCATACTGTTTCTCTGCAG(+CAGTGT)GATCCAGCACGCCTCTGGACATAGCTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605113851_3bkb GGGGGGGCACGGAGGAAAGCAAGGCTGTGTGG(+CAGTGT)GATCCACTGGAGCCTCAGGAGGAAGGGAACA NGG 10 8 17 8 24 0 100 5 10
    240605113849_fiqt CCCAGTGTTCCCTTCCTCCTGAGGCTCCAGTG(+CAGTGT)GATCCCACACAGCCTTGCTTTCCTCCGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605113847_ovyq GTATTGGTGCAGAGGAGAGCTCTTGGTGCTGG(+CAGTGT)GATCCTATGCCACTCCCCACTCCATCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240605113846_hidc TCTGTTTAGAGATGGAGTGGGGAGTGGCATAG(+CAGTGT)GATCCCAGCACCAAGAGCTCTCCTCTGCACC NGG 10 8 17 8 24 0 100 5 10
    240605113844_5nfa AAGTATAAACCTCGATTTTCCTGGGCAGACTG(+CAGTGT)GATCCTATGATCAACCTAGGAATAGGGATTA NGG 10 8 17 8 24 0 100 5 10
    240605113843_vm4u CAGTTTAATCCCTATTCCTAGGTTGATCATAG(+CAGTGT)GATCCAGTCTGCCCAGGAAAATCGAGGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605113842_mso0 TTTTATATCAGTGTGTTGAATTTTGACTTTAG(+CAGTGT)GTACCTGGAAATGTAATATCCTTGCTATAAA NGG 10 8 17 8 24 0 100 5 10
    240605113840_7b4n CATGTTTTATAGCAAGGATATTACATTTCCAG(+CAGTGT)GTACCTAAAGTCAAAATTCAACACACTGATA NGG 10 8 17 8 24 0 100 5 10
    240605113839_a9js AGTCCTTACAAGGATGGAGTGCGTGCAAAATG(+CAGTGT)GTACCTTACTGTTTCTAGAACAGCCTTCTGC NGG 10 8 17 8 24 0 100 5 10