Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113837_k3qa GAAGAGCAGAAGGCTGTTCTAGAAACAGTAAG(+CAGTGT)GTACCATTTTGCACGCACTCCATCCTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605113836_9eqy ATAAACTAGAAAACATAGATGCACAGGACTCG(+CAGTGT)GATCCATGATATTTACACTGGGAAATGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605113835_1gmt CATTTAGAACATTTCCCAGTGTAAATATCATG(+CAGTGT)GATCCGAGTCCTGTGCATCTATGTTTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605113833_9tg7 ACGATAAGCAATGGTCAGACTTTCCTTCTGTG(+CAGTGT)GATCCTTATGCCAATTCAGCTACTAATCGGT NGG 10 8 17 8 24 0 100 5 10
    240605113832_oxuy GAATGACCGATTAGTAGCTGAATTGGCATAAG(+CAGTGT)GATCCACAGAAGGAAAGTCTGACCATTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605113830_zta1 AATATGAGGGAGGCACATTTTCTAGTGGCTGG(+CAGTGT)GATCCTCTTCCAAACCATAAGAAAAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605113829_4ymw ACCACTGCCTTTTTTCTTATGGTTTGGAAGAG(+CAGTGT)GATCCCAGCCACTAGAAAATGTGCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605113827_9196 ACAGGGTTAAAGTTTTGGTCCTCAGCCCATTG(+CAGTGT)GTACCATTAGGAAACAGGGTCTAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605113826_cp52 GAAATTCTCTGACTAGACCCTGTTTCCTAATG(+CAGTGT)GTACCAATGGGCTGAGGACCAAAACTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605113825_0brd CGGACTAGGTGGTTAGTGGATACTATTGTTTG(+CAGTGT)GATCCTGACTGTTACAGGGTTAAAGTTTTGG NGG 10 8 17 8 24 0 100 5 10