Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113908_9p0f AGGGACTCTAGTCTTCAGGTTGTACTGATAAG(+CAGTGT)GATCCCAGTTTGCTGTGCAGAACACATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605113906_wg88 AGAATTATCAAGGAACACCTAAAAATGACCAG(+CAGTGT)GATCCAAATAGGTTATTTATATTATATTTCT NGG 10 8 17 8 24 0 100 5 10
    240605113904_012g TATAGAGAAATATAATATAAATAACCTATTTG(+CAGTGT)GATCCTGGTCATTTTTAGGTGTTCCTTGATA NGG 10 8 17 8 24 0 100 5 10
    240605113903_yq0z AGCTGAGTTTTGCATAATGATTTTGTATCTTG(+CAGTGT)GTACCTCACTGAATTCATTTATGATTTATGA NGG 10 8 17 8 24 0 100 5 10
    240605113902_4nlz ATCCATCATAAATCATAAATGAATTCAGTGAG(+CAGTGT)GTACCAAGATACAAAATCATTATGCAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605113900_ftur TATAATAAAATAATACTAAAAGAGAGCATGTG(+CAGTGT)GATCCATACACCAGATTAATGCGGGGATAGG NGG 10 8 17 8 24 0 100 5 10
    240605113859_mfyy CATACCCTATCCCCGCATTAATCTGGTGTATG(+CAGTGT)GATCCACATGCTCTCTTTTAGTATTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240605113857_6as6 CGACAAACGTGCATTTGATAGCACTATTTTAG(+CAGTGT)GTACCCAGAAGCAGACAATACAGCAGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605113855_3gmg GCCATATTCACTGCTGTATTGTCTGCTTCTGG(+CAGTGT)GTACCTAAAATAGTGCTATCAAATGCACGTT NGG 10 8 17 8 24 0 100 5 10
    240605113854_pmir CGTCTACTTAAGCTATGTCCAGAGGCGTGCTG(+CAGTGT)GATCCTGCAGAGAAACAGTATGGCTTTATGA NGG 10 8 17 8 24 0 100 5 10