Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114241_q1no CTGTCAGGGTTCTCTTTCCAGTGCTTCCTCGG(+CAGTGT)GTACCTGATGGAAGAGAATGTCCTGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605114239_e1jp ATGCAGGCTTCCAGGACATTCTCTTCCATCAG(+CAGTGT)GTACCCGAGGAAGCACTGGAAAGAGAACCCT NGG 10 8 17 8 24 0 100 5 10
    240605114238_akge CCCAAACTTACTCCCCCCTAAAGGAGAAAGAG(+CAGTGT)GATCCAGGAGGCTCAGTCCTGATGGAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605114236_0bcw TGTGATCCTCTCCATCAGGACTGAGCCTCCTG(+CAGTGT)GATCCTCTTTCTCCTTTAGGGGGGAGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605114234_n4dl GCCGTCGGAGTTCCCCAAATCCAGTGGGGGTG(+CAGTGT)GATCCACAATGGATCTATTCTACCTCCATAG NGG 10 8 17 8 24 0 100 5 10
    240605114233_jpda TGGACCTATGGAGGTAGAATAGATCCATTGTG(+CAGTGT)GATCCACCCCCACTGGATTTGGGGAACTCCG NGG 10 8 17 8 24 0 100 5 10
    240605114232_69a6 TTTGGCCAGGTCAGCCAGGCCTGTTTTCACAG(+CAGTGT)GATCCTAACAGGTGATCAGTGAGAAGCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605114230_ouxn GCTTCTTTAGCTTCTCACTGATCACCTGTTAG(+CAGTGT)GATCCTGTGAAAACAGGCCTGGCTGACCTGG NGG 10 8 17 8 24 0 100 5 10
    240605114229_xb25 TAGAAGAAGATGGAGACACTGTGTCCTAAGAG(+CAGTGT)GATCCTGTACCCCAGGCTAGGGTAATTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605114228_dsl9 CTGGGGGAGAATTACCCTAGCCTGGGGTACAG(+CAGTGT)GATCCTCTTAGGACACAGTGTCTCCATCTTC NGG 10 8 17 8 24 0 100 5 10