Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114328_xmzy CTCTGCCAGCCTGCTCTGTTCCTCTGTCCCTG(+CAGTGT)GATCCTCACTGGCAGCAGCTGTTACGCATTG NGG 10 8 17 8 24 0 100 5 10
    240605114326_c7u7 TGCTCCAATGCGTAACAGCTGCTGCCAGTGAG(+CAGTGT)GATCCAGGGACAGAGGAACAGAGCAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605114325_0pnr CTAGCCACACCTGTAGGGCAAGGTCACTGCAG(+CAGTGT)GTACCTCCCTGTCATTAGGCAGTGGACTTCG NGG 10 8 17 8 24 0 100 5 10
    240605114323_0l5s CTTCCCGAAGTCCACTGCCTAATGACAGGGAG(+CAGTGT)GTACCTGCAGTGACCTTGCCCTACAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605114322_i2tx ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCAATATCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605114320_o3h4 GCTGTCTTCAGACACACCAAAAGAGGATATTG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605114319_sb9o CGCCTCCCTGGTGATGCCACCTAGGAGGGTTG(+CAGTGT)GATCCTGGACTCCAGCCCACCTTCTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605114317_2bp3 CTAGGCCAGAGAGAAGGTGGGCTGGAGTCCAG(+CAGTGT)GATCCAACCCTCCTAGGTGGCATCACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605114316_ndnv CCCGAGAGCCACAGGCTGTGTCCATGAACTTG(+CAGTGT)GATCCCTGAGCACTTGAGAATGAACTCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605114314_e0s1 GAACCTCTTGAGTTCATTCTCAAGTGCTCAGG(+CAGTGT)GATCCAAGTTCATGGACACAGCCTGTGGCTC NGG 10 8 17 8 24 0 100 5 10