Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112630_hclw CCTGTGCCCACTGGAGCCAGAAGAGGGCATTG(+CAGTGT)GATCCTGAAACTGAAGTTACAGATGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605112629_m47k GTGGCTCCCAACATCTGTAACTTCAGTTTCAG(+CAGTGT)GATCCAATGCCCTCTTCTGGCTCCAGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605112627_rd26 AGGGCTGAGAAGGTAGGAGCAGTACTGCAGGG(+CAGTGT)GATCCTAAGCCAGAGGTCTGGTTAATTTACA NGG 10 8 17 8 24 0 100 5 10
    240605112626_ptpo TATGGTGTAAATTAACCAGACCTCTGGCTTAG(+CAGTGT)GATCCCCTGCAGTACTGCTCCTACCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605112625_2n61 TCCTCTGAACTCTCTCATCTACCTGAAAACTG(+CAGTGT)GATCCACAGAACAGTTAACTTTGATCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605112623_ckba CTCAGGGTGGGATCAAAGTTAACTGTTCTGTG(+CAGTGT)GATCCAGTTTTCAGGTAGATGAGAGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605112622_ar2f ACCAGTGTTCTTTATACACTCTATTCCAGGTG(+CAGTGT)GATCCTTTGACATTTACGTGGTTTGGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605112620_20so ATGCCACCAACCAAACCACGTAAATGTCAAAG(+CAGTGT)GATCCACCTGGAATAGAGTGTATAAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605112619_xzyo AGTCCAAAAGTCACTCTGAGATCCAAGCTTCG(+CAGTGT)GATCCCCCAGACAGTGGCATCACCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605112617_keo6 ATGCAAGACAGAGGTGATGCCACTGTCTGGGG(+CAGTGT)GATCCGAAGCTTGGATCTCAGAGTGACTTTT NGG 10 8 17 8 24 0 100 5 10