Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112545_6r0u GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+CAGTGT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605112542_c6k7 GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+CAGTGT)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605112541_gh6i CTTTGGGGACCAACCTTGTTGCGTAGTGTGTG(+CAGTGT)GTACCAGCTTTGCGCTTTGCCTGGGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605112539_vjvv GATACAGTTTTCCCAGGCAAAGCGCAAAGCTG(+CAGTGT)GTACCACACACTACGCAACAAGGTTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605112538_ql2v GGTCGGACTTGTGACTTAGGCTTGCAGCCTTG(+CAGTGT)GATCCTGGGGTTTGCACTGATATGGTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605112535_c1sk GGATGACAGAACCATATCAGTGCAAACCCCAG(+CAGTGT)GATCCAAGGCTGCAAGCCTAAGTCACAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605112533_jvt1 CATAGCATTCACCATGATCATATGCCCCTCGG(+CAGTGT)GATCCTGAGAGAAGAGAGAGGCTTGTACTGA NGG 10 8 17 8 24 0 100 5 10
    240605112531_vdgy ACCCCTCAGTACAAGCCTCTCTCTTCTCTCAG(+CAGTGT)GATCCCGAGGGGCATATGATCATGGTGAATG NGG 10 8 17 8 24 0 100 5 10
    240605112530_3s0a GATAGACATTATCTCCCCCCCCCCTTTTTCAG(+CAGTGT)GTACCTGTAATTTGTGTCTGAGAGGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605112529_aee1 TTCCCACCCTGCCTCTCAGACACAAATTACAG(+CAGTGT)GTACCTGAAAAAGGGGGGGGGGAGATAATGT NGG 10 8 17 8 24 0 100 5 10