Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112528_s0nz GGTGTCATCGTTTGGAGGCTGATTATGGGATG(+CAGTGT)GATCCCCGGGTATGGCAGTCTCAGAATACTT NGG 10 8 17 8 24 0 100 5 10
    240605112524_5u6c GATAAAAGTATTCTGAGACTGCCATACCCGGG(+CAGTGT)GATCCATCCCATAATCAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240605112523_xolt AGGTATTCCCCGGTCCCTACAGCTAATCCATG(+CAGTGT)GATCCCTGAAAAGGGACTGCAGGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605112521_sanj CAGTACTGTCCTGCCTGCAGTCCCTTTTCAGG(+CAGTGT)GATCCATGGATTAGCTGTAGGGACCGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605112519_bjx8 TTTCATAACTTCAATAGCTTTTTTCTTTTCGG(+CAGTGT)GATCCCAGTGTTGGCTCTATTTCACTTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605112518_sme4 ATTATTAGAAAGTGAAATAGAGCCAACACTGG(+CAGTGT)GATCCCGAAAAGAAAAAAGCTATTGAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605112516_0bb7 GTTTTCTCCTCCAACGAAAACCTCAAGTTCAG(+CAGTGT)GTACCACTGAAAAGAGACTAGTTTGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605112515_e299 TAGTCTCTTTTCAAACTAGTCTCTTTTCAGTG(+CAGTGT)GTACCTGAACTTGAGGTTTTCGTTGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605112514_jjo7 GAATGTTAGATATTTTTTCCATGGGGTCAAAG(+CAGTGT)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605112513_3jb8 CCCTATGTTTCCACTCGCAATCATATACTTAG(+CAGTGT)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10