Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112455_qda0 CATGCAATAAATGACCATTACAGGTTTCAAAG(+CAGTGT)GTACCCTAGGTTTTCTAGGTGAGAGTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605112452_hjtb GTGGGTGTGAACTCTCACCTAGAAAACCTAGG(+CAGTGT)GTACCTTTGAAACCTGTAATGGTCATTTATT NGG 10 8 17 8 24 0 100 5 10
    240605112450_ovdk GGGCTGAGTGGAGACCCAGTTGGAGGAGGCTG(+CAGTGT)GATCCTTTGTTAGGAAGGCAAAGGCCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605112449_pug1 AGGTTCCCCGGGCCTTTGCCTTCCTAACAAAG(+CAGTGT)GATCCAGCCTCCTCCAACTGGGTCTCCACTC NGG 10 8 17 8 24 0 100 5 10
    240605112448_pbky TTATCAGCTTTCATTCAGTCTTCCTTTCTCTG(+CAGTGT)GATCCAATTATCTCACCTCCTTTAGCTATTC NGG 10 8 17 8 24 0 100 5 10
    240605112447_3eg0 TTATGGAATAGCTAAAGGAGGTGAGATAATTG(+CAGTGT)GATCCAGAGAAAGGAAGACTGAATGAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605112445_cm5l CTACAATTCTATTTTAATCAGCTTTTTGGCTG(+CAGTGT)GTACCTACTGCTATACCACCTAGTATGACAT NGG 10 8 17 8 24 0 100 5 10
    240605112444_h6ix CCGAAATGTCATACTAGGTGGTATAGCAGTAG(+CAGTGT)GTACCAGCCAAAAAGCTGATTAAAATAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605112442_mrl1 TTTTTTATTCCTTCTCTGGCAATTCTTTCCTG(+CAGTGT)GATCCTCTGCTTAGCGAAATTATACCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605112441_2apr TTCCACTGTAGGTATAATTTCGCTAAGCAGAG(+CAGTGT)GATCCAGGAAAGAATTGCCAGAGAAGGAATA NGG 10 8 17 8 24 0 100 5 10