Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112511_l1gt TAGTGACCGGTGCACACTGCAGCTACCCAGTG(+CAGTGT)GTACCCCTGGAAGGGACCTGTGATGTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605112508_r88u TTTAACTTTGACATCACAGGTCCCTTCCAGGG(+CAGTGT)GTACCACTGGGTAGCTGCAGTGTGCACCGGT NGG 10 8 17 8 24 0 100 5 10
    240605112506_bfgt GGACTCCTCAGACCATGGCTGTGTGCAGCTGG(+CAGTGT)GTACCAGAATCCTGTGCTCAGTCTCTGTTCG NGG 10 8 17 8 24 0 100 5 10
    240605112505_e01b CAACACGAACAGAGACTGAGCACAGGATTCTG(+CAGTGT)GTACCCAGCTGCACACAGCCATGGTCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605112503_j0dv ATCATGGAAGGCATGCCAAGTCCACCCGCTGG(+CAGTGT)GATCCAGCGGGGCACAGTACAGTTCCTCCGG NGG 10 8 17 8 24 0 100 5 10
    240605112502_4tbw AGGAGCCGGAGGAACTGTACTGTGCCCCGCTG(+CAGTGT)GATCCCAGCGGGTGGACTTGGCATGCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605112500_qz20 ACTAGGCAGAGCAAACATCAGGAAGTGGGAAG(+CAGTGT)GTACCGATATGAAATTAAGAGAAAGGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240605112459_sk7d TTTGAGTCGGCCTTTCTCTTAATTTCATATCG(+CAGTGT)GTACCTTCCCACTTCCTGATGTTTGCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605112457_zy5x CCCTTGGTCCCTAATCAGTGCCTGCTGGCGTG(+CAGTGT)GATCCCAGAGGAGCCTCTGTACATGCCATCC NGG 10 8 17 8 24 0 100 5 10
    240605112456_jcgw ACTCTGGATGGCATGTACAGAGGCTCCTCTGG(+CAGTGT)GATCCACGCCAGCAGGCACTGATTAGGGACC NGG 10 8 17 8 24 0 100 5 10