Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112347_e22o TATGTGTGGGGATCCCTTCACCCACCATACTG(+CAGTGT)GTACCCCTTCTTCAGCTCCATCTGTCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605112346_do0f AGGGGATAGGGACAGATGGAGCTGAAGAAGGG(+CAGTGT)GTACCAGTATGGTGGGTGAAGGGATCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605112344_dl0f CTTCTTCTTTCTCTAGTGCAAGTATGTGTGGG(+CAGTGT)GATCCCTTCACCCACCATACTGGTACCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605112342_o9xl TGAAGAAGGGGTACCAGTATGGTGGGTGAAGG(+CAGTGT)GATCCCCACACATACTTGCACTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605112340_cdk3 TATTTGCTGTGCAAAGCACGCCCTAAGATGGG(+CAGTGT)GTACCTTTGATCTCACCGTGCGGCTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605112339_c1m1 TTGAATCCTGCAGCCGCACGGTGAGATCAAAG(+CAGTGT)GTACCCCATCTTAGGGCGTGCTTTGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240605112337_ka6c GAAATAGGGAACACCCACATCTTTCTTTCTGG(+CAGTGT)GATCCATGCGGATGACTGTCCTGGTGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605112336_7md5 CCATCGAAGCCACCAGGACAGTCATCCGCATG(+CAGTGT)GATCCCAGAAAGAAAGATGTGGGTGTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605112335_v8oc TAGATATAATGATGTCTAGGACAGATAAAAAG(+CAGTGT)GATCCACTTATTGGCACTTGCCATGATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605112334_nkrs CTGAACAGGATCATGGCAAGTGCCAATAAGTG(+CAGTGT)GATCCTTTTTATCTGTCCTAGACATCATTAT NGG 10 8 17 8 24 0 100 5 10