Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112332_zn6e CCTGTTGGGACTTGATGTGCCAGGGTGGGTTG(+CAGTGT)GTACCGTCTCTGAGGAGAAGGGGAGATGGGA NGG 10 8 17 8 24 0 100 5 10
    240605112331_xn52 CCAATTCCCATCTCCCCTTCTCCTCAGAGACG(+CAGTGT)GTACCAACCCACCCTGGCACATCAAGTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605112329_qsb6 ATCAGGATGATGTACACACTGGGTGATTCTTG(+CAGTGT)GATCCAGAGATCTGTTTGAACTGCACCATCT NGG 10 8 17 8 24 0 100 5 10
    240605112328_lgy7 AAATTAGATGGTGCAGTTCAAACAGATCTCTG(+CAGTGT)GATCCAAGAATCACCCAGTGTGTACATCATC NGG 10 8 17 8 24 0 100 5 10
    240605112326_r2nw AGTTTGCTGAACATTTGACAGGGACAAGGAGG(+CAGTGT)GATCCATACGACAGCAGTTCCCGCTGTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605112325_kvx6 CTTCATTCCACAGCGGGAACTGCTGTCGTATG(+CAGTGT)GATCCCTCCTTGTCCCTGTCAAATGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605112323_qljq AGTTTGCTGAACATTTGACAGGGACAAGGAGG(+CAGTGT)GATCCATACGACAGCAGTTCCCGCTGTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605112322_l1mg CTTCATTCCACAGCGGGAACTGCTGTCGTATG(+CAGTGT)GATCCCTCCTTGTCCCTGTCAAATGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605112320_m54m GCATAGCCTTCTTCTGTGGGTTACCTCCTCAG(+CAGTGT)GTACCCAGGTCACATCCTGGTTAAGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605112318_c79i CAAGGTGAGAACTTAACCAGGATGTGACCTGG(+CAGTGT)GTACCTGAGGAGGTAACCCACAGAAGAAGGC NGG 10 8 17 8 24 0 100 5 10