Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112810_b57a TTTCAAGAGTTTAAAGTTTCAAACCAGGGAAG(+CAGTGT)GATCCAAGATAAAAAGATGACACATCAGACA NGG 10 8 17 8 24 0 100 5 10
    240605112808_l64o CCTGTCCTTTAATTCTCAGAACCCCTTTCGAG(+CAGTGT)GTACCTACATTGACTGGTAGTCGAGGGTCGA NGG 10 8 17 8 24 0 100 5 10
    240605112807_bbsp TGTAGTCGACCCTCGACTACCAGTCAATGTAG(+CAGTGT)GTACCTCGAAAGGGGTTCTGAGAATTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605112806_qjdf TGCTTCTGCCAAATGACTAATATGGCCTGCTG(+CAGTGT)GATCCCGAGCACACGGGGGCTTTGAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605112804_2rqw TGAGTGTGACCTCAAAGCCCCCGTGTGCTCGG(+CAGTGT)GATCCAGCAGGCCATATTAGTCATTTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605112802_ppku TTAACTTTTCCCAGGGGTCCAGCACCTCACCG(+CAGTGT)GATCCACCTAAGGGCGCTTACTAAAAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605112801_2z1n AGATGCCACCTTTTAGTAAGCGCCCTTAGGTG(+CAGTGT)GATCCGGTGAGGTGCTGGACCCCTGGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605112759_ic7b AAGAAATCAGCAATCAGTAGTCCTCCCCTGAG(+CAGTGT)GATCCTCGGAACCTCCCTCTAGGCAAATCAA NGG 10 8 17 8 24 0 100 5 10
    240605112757_qdly GGGGCTTGATTTGCCTAGAGGGAGGTTCCGAG(+CAGTGT)GATCCTCAGGGGAGGACTACTGATTGCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605112756_79wv TAGGGAAGCTGGAGAGCAGGTGTGAACAGAAG(+CAGTGT)GATCCCTAGACTTCAGCAGCAGGTTCGCAGG NGG 10 8 17 8 24 0 100 5 10