Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112725_nqa8 GGTAGACACTTCTGCCTGAATCCCATTAGTGG(+CAGTGT)GATCCTGCTTTGCATAGGACCCCAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605112723_axmo CGACTCAAAGGGCCTTCTTGCTGGGGCCTCTG(+CAGTGT)GATCCTCTGTCCTGGGGCCGGAAGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605112722_uuvy TCCGGCCCCTGCCTTCCGGCCCCAGGACAGAG(+CAGTGT)GATCCAGAGGCCCCAGCAAGAAGGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605112721_r365 TTGTCATTCATCACGAAGTAGGCCAAATCATG(+CAGTGT)GTACCACAGGAATTTAGAAATCTAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605112718_v8ec CCAGATTGCAGTTAGATTTCTAAATTCCTGTG(+CAGTGT)GTACCATGATTTGGCCTACTTCGTGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605112717_jyor AAAGCCCTGGGTTGACTGACTCCCTCACTCAG(+CAGTGT)GATCCTAAGAACAATGCTGACTGCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605112716_fhmj AAGTAGAAAACTGCAGTCAGCATTGTTCTTAG(+CAGTGT)GATCCTGAGTGAGGGAGTCAGTCAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605112714_s3d0 GTGCAAGCGCCATGGCACCCTGCTGTACCACG(+CAGTGT)GTACCAAACTCAACTGTACAGTCAACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605112713_j8e1 CTCGCTGACTGTTGACTGTACAGTTGAGTTTG(+CAGTGT)GTACCGTGGTACAGCAGGGTGCCATGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240605112712_t0sq CGTAGGCCGATGAGTGTAAGTAAAAGAGTGTG(+CAGTGT)GTACCACTGGAAAAAACTCCAAACCAGGGCC NGG 10 8 17 8 24 0 100 5 10