Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112101_6usq CAGATTTTAGGTAAGTTTATTTGACTAAATTG(+CAGTGT)GATCCTTTTATATTAACAATTCAGCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240605112100_fp7r TTATAAAAGATGCTGAATTGTTAATATAAAAG(+CAGTGT)GATCCAATTTAGTCAAATAAACTTACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605112059_bypk ATTCCTGTCCCAGGATCCAAATCCCTCTTCAG(+CAGTGT)GTACCAGGCACTGTGCAAAGACACATTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605112057_9mum TTTTGCCTGAATGTGTCTTTGCACAGTGCCTG(+CAGTGT)GTACCTGAAGAGGGATTTGGATCCTGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605112055_nhzm AGCTCACAACTGTCTTGTTATTCCTGTCCCAG(+CAGTGT)GATCCAAATCCCTCTTCAGGTACCAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240605112054_t0t9 TGCACAGTGCCTGGTACCTGAAGAGGGATTTG(+CAGTGT)GATCCTGGGACAGGAATAACAAGACAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605112051_xmp6 TATATAATATCTTCTTTTTTTTTAAAGCCCCG(+CAGTGT)GATCCAAGGCGTCCAAAGTTATTAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605112049_tr68 TGAACTTTATTTTAATAACTTTGGACGCCTTG(+CAGTGT)GATCCGGGGCTTTAAAAAAAAAGAAGATATT NGG 10 8 17 8 24 0 100 5 10
    240605112048_2s19 GTAAGATACATAAATATAGAATTAATATAAAG(+CAGTGT)GTACCACTATGTTTTACTTATAATAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240605112047_u23k AAATGTGTTTTTATTATAAGTAAAACATAGTG(+CAGTGT)GTACCTTTATATTAATTCTATATTTATGTAT NGG 10 8 17 8 24 0 100 5 10