Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112045_vqy9 CAACTGAGAGAAGTGGGAAGAGCATCTGGTGG(+CAGTGT)GTACCAATTGGCAGAGAAGGGGAGAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605112044_tkvh ATGACCAGTCTTCTCCCCTTCTCTGCCAATTG(+CAGTGT)GTACCCACCAGATGCTCTTCCCACTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605112043_u376 TCTCTTCCACTCAGAAAGTGGCATGGCTTCAG(+CAGTGT)GTACCACAGGGCACATATGTCAATCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240605112041_p4m7 AGACCAGGGTTGATTGACATATGTGCCCTGTG(+CAGTGT)GTACCTGAAGCCATGCCACTTTCTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605112039_3f1u CCCATGAGAGTGTCGGGGTCAGGGGCTGAAAG(+CAGTGT)GATCCAACATTTCCAAGTTAGAAGTTAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605112038_dnlr GTTAAAGCTTAACTTCTAACTTGGAAATGTTG(+CAGTGT)GATCCTTTCAGCCCCTGACCCCGACACTCTC NGG 10 8 17 8 24 0 100 5 10
    240605112037_102c TGGCAGGATCAACATGTGTAACTGCCTTCTTG(+CAGTGT)GTACCTGTTTGATTAGTGGGTAGAAAATAGC NGG 10 8 17 8 24 0 100 5 10
    240605112036_sqju TAACTGCTATTTTCTACCCACTAATCAAACAG(+CAGTGT)GTACCAAGAAGGCAGTTACACATGTTGATCC NGG 10 8 17 8 24 0 100 5 10
    240605112034_2kho AAGAGGTCACACGGTTTGGAAACTTGGGGTAG(+CAGTGT)GTACCAGAGCATACAAGTTTAAATCAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605112033_xwik TGCAATGCCCTGATTTAAACTTGTATGCTCTG(+CAGTGT)GTACCTACCCCAAGTTTCCAAACCGTGTGAC NGG 10 8 17 8 24 0 100 5 10