Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111942_hmpj TAGGCCCCTCCCAGCTGTGAGGGTCCAGGGAG(+CAGTGT)GATCCTGTGGCCGAGTCTTCAGTGGCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240605111940_or08 GCTTTACTCGGCCACTGAAGACTCGGCCACAG(+CAGTGT)GATCCTCCCTGGACCCTCACAGCTGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605111939_0749 TGTCAGGAAAAACACATGCCCATGAAGGAATG(+CAGTGT)GTACCCTCTAGTACCACCAAGCTCCACATGA NGG 10 8 17 8 24 0 100 5 10
    240605111937_th50 ATGGCTCATGTGGAGCTTGGTGGTACTAGAGG(+CAGTGT)GTACCATTCCTTCATGGGCATGTGTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605111935_t9t8 ACTGAAATGGGAATGTTCCCAAGGGGCCTGGG(+CAGTGT)GATCCCCTGTGAGGAGAAGTCCTTGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605111933_1xpg AGCTTCAGGCTCAAGGACTTCTCCTCACAGGG(+CAGTGT)GATCCCCAGGCCCCTTGGGAACATTCCCATT NGG 10 8 17 8 24 0 100 5 10
    240605111931_kd77 GGTGGATATCCTGTAACATAAATGCTCGTTTG(+CAGTGT)GATCCAAAAGGAAAACCATTAAAAATAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605111930_5tuq TCTTCCTTTTATTTTTAATGGTTTTCCTTTTG(+CAGTGT)GATCCAAACGAGCATTTATGTTACAGGATAT NGG 10 8 17 8 24 0 100 5 10
    240605111929_dkwc TAACGTTGACACAAACACAGATAAGCTCGTGG(+CAGTGT)GTACCTGGACAAAAGGGTGCCTTAGTTTATG NGG 10 8 17 8 24 0 100 5 10
    240605111927_bp0w GAGGACATAAACTAAGGCACCCTTTTGTCCAG(+CAGTGT)GTACCCACGAGCTTATCTGTGTTTGTGTCAA NGG 10 8 17 8 24 0 100 5 10