Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111925_lueo GTGACAAGTTAGAATGTCTCTCTTGGTTTGTG(+CAGTGT)GTACCCCCAGGTTGGCTTTGTTCTGTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605111923_7n7c GGACTTTTCCACAGAACAAAGCCAACCTGGGG(+CAGTGT)GTACCACAAACCAAGAGAGACATTCTAACTT NGG 10 8 17 8 24 0 100 5 10
    240605111922_ayto TCTGCTGGCATACCACATGACACAAAGCAGAG(+CAGTGT)GATCCCAGCAAACTAGTGGGATCTCGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605111920_haws GGGCCCATTCCGAGATCCCACTAGTTTGCTGG(+CAGTGT)GATCCTCTGCTTTGTGTCATGTGGTATGCCA NGG 10 8 17 8 24 0 100 5 10
    240605111919_6wtu AGTGGCACAGACGCCCAGCGCCAGGAGTATTG(+CAGTGT)GTACCACCTCTCCTGTTTTAACTGGAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605111917_ex40 TCCATCCCCTTCCAGTTAAAACAGGAGAGGTG(+CAGTGT)GTACCAATACTCCTGGCGCTGGGCGTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605111915_p46t GGTGTGTTTCCAGGAGCCAGCACCCCACCAGG(+CAGTGT)GTACCGCATAAACATTGCAGGAGAGTTTCCG NGG 10 8 17 8 24 0 100 5 10
    240605111914_70ju GGCTGCGGAAACTCTCCTGCAATGTTTATGCG(+CAGTGT)GTACCCTGGTGGGGTGCTGGCTCCTGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605111913_glwr GTCAGGCTTTTCCCTCAGAAGATCACAGTTTG(+CAGTGT)GATCCTGAGAGGAATCTGCATGGGCTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605111912_gog1 GAGAGGCTGAAGCCCATGCAGATTCCTCTCAG(+CAGTGT)GATCCAAACTGTGATCTTCTGAGGGAAAAGC NGG 10 8 17 8 24 0 100 5 10