Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111855_ypu8 CTCCAAAGCTAGAGTAATCATGAGGGAGGCAG(+CAGTGT)GATCCACGGAGAATCACAAAGGATGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240605111853_6zok GTATCAAAATGCATCCTTTGTGATTCTCCGTG(+CAGTGT)GATCCTGCCTCCCTCATGATTACTCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605111852_7pp8 GTATGCTGAGGTGTGGGGGTGGTGAATGAAGG(+CAGTGT)GTACCCTACCTGCAGTGCTCAAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605111850_s68q ATCATTGGACATTCTTGAGCACTGCAGGTAGG(+CAGTGT)GTACCCTTCATTCACCACCCCCACACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605111849_td4n TTAATCCCAGCACTAAGGAGGCAGAGCAGGCG(+CAGTGT)GATCCCAGTGAGTTTGAAGCCTGAGCTATAG NGG 10 8 17 8 24 0 100 5 10
    240605111847_o10w TCATCCTATAGCTCAGGCTTCAAACTCACTGG(+CAGTGT)GATCCGCCTGCTCTGCCTCCTTAGTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605111846_cq88 GGACAGAAGCAGTTTGAGCAGGTGTCTATATG(+CAGTGT)GTACCCTGCATCCGGGGCCCTTTGGGAACTG NGG 10 8 17 8 24 0 100 5 10
    240605111844_i3kn CCGGCCAGTTCCCAAAGGGCCCCGGATGCAGG(+CAGTGT)GTACCATATAGACACCTGCTCAAACTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605111843_dfjt CACATTTCTAAAAATCTTTCAATGACAAGTTG(+CAGTGT)GTACCCTTTAGCTAACTAAAGCTAAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605111841_tqh4 CCTTCCACCTTTTAGCTTTAGTTAGCTAAAGG(+CAGTGT)GTACCAACTTGTCATTGAAAGATTTTTAGAA NGG 10 8 17 8 24 0 100 5 10