Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112152_bg91 CTCTTTTCCTTCTCTTATTCATTTGAGTGGTG(+CAGTGT)GTACCTTATTCTGTGAGAATAATGCTCATGG NGG 10 8 17 8 24 0 100 5 10
    240605112151_2s04 GTTCCCCATGAGCATTATTCTCACAGAATAAG(+CAGTGT)GTACCACCACTCAAATGAATAAGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605112149_p9ir GTGTTGTTGTTGTAGTTTGTTTCTATGCCGTG(+CAGTGT)GTACCGTGGTTATCTCCCATGGAGGACTCCG NGG 10 8 17 8 24 0 100 5 10
    240605112147_8dzp CAGCCCGGAGTCCTCCATGGGAGATAACCACG(+CAGTGT)GTACCACGGCATAGAAACAAACTACAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605112145_lu76 CGAGGATACCCGAATGTGATGAAGGGCTCAGG(+CAGTGT)GTACCAGTGATGGGGCCACTGGAGACATCTC NGG 10 8 17 8 24 0 100 5 10
    240605112144_jeyy CCAAGGAGATGTCTCCAGTGGCCCCATCACTG(+CAGTGT)GTACCCTGAGCCCTTCATCACATTCGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605112143_2327 GTCACAGGGCTGGCATCTGGAACACAGCACAG(+CAGTGT)GTACCAAGACAAGGGTAAAATCTTGTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605112141_lozx CTGCTGCTTTACAAGATTTTACCCTTGTCTTG(+CAGTGT)GTACCTGTGCTGTGTTCCAGATGCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605112140_sls3 TTTTGAGACACAGGTTTTCTATGGCCTTCCTG(+CAGTGT)GATCCTCATTGTCTCTCTCCCCAACTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605112138_xtn5 CTGTTCTGTGAGTTGGGGAGAGAGACAATGAG(+CAGTGT)GATCCAGGAAGGCCATAGAAAACCTGTGTCT NGG 10 8 17 8 24 0 100 5 10