Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111013_zvj8 TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+CAGTGT)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240605111012_6r5f GAAGGCTACATGGAGCCCTCACAATAAAAATG(+CAGTGT)GTACCTTTTTTTTTTGAGACAGGCTCTTACT NGG 10 8 17 8 24 0 100 5 10
    240605111011_ipo4 GGGCTAGTGTACAAGCACAGCCAGAGTCGTCG(+CAGTGT)GTACCAGCACTTCTCTATTTACTCTATTGGA NGG 10 8 17 8 24 0 100 5 10
    240605111009_ozd4 TCCGCTCCAATAGAGTAAATAGAGAAGTGCTG(+CAGTGT)GTACCGACGACTCTGGCTGTGCTTGTACACT NGG 10 8 17 8 24 0 100 5 10
    240605111008_91q7 TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+CAGTGT)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240605111007_esj0 GAAGGCTACATGGAGCCCTCACAATAAAAATG(+CAGTGT)GTACCTTTTTTTTTTGAGACAGGCTCTTACT NGG 10 8 17 8 24 0 100 5 10
    240605111006_lw4k GGGCTAGTGTACAAGCACAGCCAGAGTCGTCG(+CAGTGT)GTACCAGCACTTCTCTATTTACTCTATTGGA NGG 10 8 17 8 24 0 100 5 10
    240605111004_sxpp TCCGCTCCAATAGAGTAAATAGAGAAGTGCTG(+CAGTGT)GTACCGACGACTCTGGCTGTGCTTGTACACT NGG 10 8 17 8 24 0 100 5 10
    240605111003_kpdo TTCAGGATAGTATGGGAACAAAGAAAGAGAAG(+CAGTGT)GTACCTCTATCCCTGTATCTCCTTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605111002_7a7j GGTGTTCCTTTGAAGGAGATACAGGGATAGAG(+CAGTGT)GTACCTTCTCTTTCTTTGTTCCCATACTATC NGG 10 8 17 8 24 0 100 5 10