Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110928_rfbd CCCTGGTGGACTCTAAAGGAACATTTGCTCAG(+CAGTGT)GTACCTGAGCTAGACCTGGCCCCAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605110927_65c5 ACTTGTTAAGAGGCAAGGCCACCTTTCACCAG(+CAGTGT)GTACCCTGGCCAGGCACCTGGGAAGACTCTT NGG 10 8 17 8 24 0 100 5 10
    240605110924_3jh4 GCCAGAAGAGTCTTCCCAGGTGCCTGGCCAGG(+CAGTGT)GTACCTGGTGAAAGGTGGCCTTGCCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605110922_o076 TCCTGGGCAGCCTGGAGGGCTGGCATGCCTGG(+CAGTGT)GATCCTCGGCCACCCAGGATCTTGGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605110920_uvi5 GACACTTGTCTCCAAGATCCTGGGTGGCCGAG(+CAGTGT)GATCCCAGGCATGCCAGCCCTCCAGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605110918_3soa GCCAGGACAGTCTTCTTCAGCTGGGCAGGCGG(+CAGTGT)GATCCTGGGAAAGCGAAGTGGCAGAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605110915_ycn6 CAATGCCAGCTTCTGCCACTTCGCTTTCCCAG(+CAGTGT)GATCCCGCCTGCCCAGCTGAAGAAGACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605110914_uakr TCACACAATAAGATTAAGTTTTAGAAACTAAG(+CAGTGT)GATCCCACCTTTTTTTCTTGTTTAAAATAAT NGG 10 8 17 8 24 0 100 5 10
    240605110912_nav7 AATAAATTATTTTAAACAAGAAAAAAAGGTGG(+CAGTGT)GATCCTTAGTTTCTAAAACTTAATCTTATTG NGG 10 8 17 8 24 0 100 5 10
    240605110910_ghrb AGGATACCATGGTCTCCAGGGGCTAAGAGAAG(+CAGTGT)GTACCTCTCTCAAGGGAAGGAAAACACAGTC NGG 10 8 17 8 24 0 100 5 10